HILIC

SPECMA Si (USP L3)

Specma Si columns for Normal Phase and HILIC applications

Unmodified Silica gel materials were the first ones used for chromatography. This is the reason the mode is called "Normal phase" (NP). They are still used in HPLC, although Reversed Phase (NP) is by far the most often used HPLC mode today. A wide range of porous Si materials is available, characterized by:

• Surface area
• Pore size
• Particle size
• Purity
• Metal content

SPECMA Amino / NH2 (USP L8)

Specma Amino columns for Normal Phase and HILIC applications

Amino (NH2) bonded silica gel phases can be used ideally in the normal phase and HILIC mode. They show alternative normal-phase selectivity to unbonded silica, especially for aromatics.

Amino columns can also be used in the HILIC mode for carbohydrate analysis and for other polar compounds. Their weak anion-exchange properties can be used in the analysis of anions and organic acids

Typically, the amino group is bonded to the silica support via a propyl linker. Most Amino phases shown here come without endcapping, only ProntoSIL Amino K is a fully end capped amino propyl bonded phase

SPECMA Zwitterionic HILIC

Specma Zwitterionic and polar columns for HILIC applications

Based on ultra-pure Silica gel, zwitterionic HILIC phases are ideal for the analysis of polar and ionizable compounds. Stronger interaction of more polar compounds with stationary aqueous layer.

Faster elution of nonpolar compounds is possible due to minor hydrophobic interactions. Retention increases with the charge of the analyte. Recommended for the analysis of hydrophilic compounds like:

• Polar organic acids and bases
• Natural polar compounds
• Nucleosides
• Oligonucleotides
• Amino acids
• Peptides
• Water soluble vitamins